Summary
Catalytic residues are usually highly destabilizing (refs 25 and 30 of (1)).
See also
- Stability-activity trade-off during enzyme design and evolution is highly local and not global
- Mutations that give rise to new functions are not more destabilizing than mutations in general
1.
Teufl M, Zajc CU, Traxlmayr MW. Engineering Strategies to Overcome the Stability–Function Trade-Off in Proteins. ACS Synthetic Biology. 2022;11(3):1030–9. Available from: https://doi.org/10.1021/acssynbio.1c00512