Summary
The thermostability and catalytic activity values of consensus sequences are sensitive to the breadth of the sequences from which they are calculated (1,2). Consensus sequences of adenylate kinase generated from one fitness peak were found to be more active than those generated from the full distribution of sequences.
Figures
Ref (2)
See also
1.
Nixon C, Lim SA, Sternke M, Barrick D, Harms MJ, Marqusee S. The importance of input sequence set to consensus‐derived proteins and their relationship to reconstructed ancestral proteins. Protein Science. 2024;33(6). Available from: https://doi.org/10.1002/pro.5011
2.
Muir DF, Asper GPR, Notin P, Posner JA, Marks DS, Keiser MJ, et al. Evolutionary-scale enzymology enables exploration of a rugged catalytic landscape. Science. 2025;388(6752). Available from: https://doi.org/10.1126/science.adu1058