Summary
Cooling samples for cryogenic X-ray crystallography or cryo-EM narrows the conformational ensemble (ref 22 of (1)). This has also been observed using spin labels for (DEER) (2).
1.
Rakotoharisoa RV, Seifinoferest B, Zarifi N, Miller JDM, Rodriguez JM, Thompson MC, et al. Design of Efficient Artificial Enzymes Using Crystallographically Enhanced Conformational Sampling. Journal of the American Chemical Society. 2024;146(14):10001–13. Available from: https://doi.org/10.1021/jacs.4c00677
2.
Georgieva ER, Roy AS, Grigoryants VM, Borbat PP, Earle KA, Scholes CP, et al. Effect of freezing conditions on distances and their distributions derived from Double Electron Electron Resonance (DEER): A study of doubly-spin-labeled T4 lysozyme. Journal of Magnetic Resonance. 2012;216:69–77. Available from: https://doi.org/10.1016/j.jmr.2012.01.004