DNA sequence entropy is strongly predictive of protein expression

Summary

DNA sequence entropy is broadly predictive of protein expression (1). Poor expressors tended to have highly unusual DNA sequences.

See also

• Glutamate- and lysine-rich designs can fail due to expression failures resulting from adenosine-rich sequences

1.

Kosonocky CW, Abel AM, Feller AL, Cifuentes Rieffer AE, Woolley PR, Lála J, et al. Validation and analysis of 12,000 AI-driven CAR-T designs in the Bits to Binders competition. openRxiv; 2026. Available from: https://doi.org/10.64898/2026.03.03.709355