Helicase-assisted continuous editing (HACE) is a method similar to CRISPR that introduces random mutations at predetermined points in a genome (1).
Details
- Cas9 introduces single-stranded DNA breaks (as opposed to double-stranded breaks)
- HACE editor is a fusion of helicase and a base editing enzyme
- Guide RNA (sgRNA) recruits enzyme to specific sequences
- HACE loads onto site of nick and drags base editor to make new mutations in genome
Figures
Figure 1 from Chen et al. (1)
1.
Chen XD, Chen Z, Wythes G, Zhang Y, Orr BC, Sun G, et al. Helicase-assisted continuous editing for programmable mutagenesis of endogenous genomes. Science. 2024;386(6718). Available from: https://doi.org/10.1126/science.adn5876