Summary
The mega-scale dataset (1) is inaccurate for DNA-binding proteins (2).
1.
Tsuboyama K, Dauparas J, Chen J, Laine E, Mohseni Behbahani Y, Weinstein JJ, et al. Mega-scale experimental analysis of protein folding stability in biology and design. Nature. 2023;620(7973):434–44. Available from: https://doi.org/10.1038/s41586-023-06328-6
2.
Ferrari ÁJR, Dixit SM, Thibeault J, Garcia M, Houliston S, Ludwig RW, et al. Large-scale discovery, analysis, and design of protein energy landscapes. openRxiv; 2025. Available from: https://doi.org/10.1101/2025.03.20.644235