Summary
Antibodies and nanobodies can de-symmetrize large symmetric proteins for cryo-EM studies. (1) used a conformationally non-selective nanobody to de-symmetrize the large primary active transporter TmrAB to assist with cryo-EM.
1.
Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Brüchert S, et al. Conformation space of a heterodimeric ABC exporter under turnover conditions. Nature. 2019;571(7766):580–3. Available from: https://doi.org/10.1038/s41586-019-1391-0