Glycosylation refers to the post-translation addition of sugars to amino acids. Antibodies are naturally glycosylated but as therapeutics are sensitive to the addition of more sugar groups. N-linked glycosylation sites include NG and N-!P-S/T-N where (!P is not Pro) (1), while O-linked glycosylation sites include T-V-X-P and S/T-P-X-P where X is anything** (2).
1.
Tennenhouse A, Khmelnitsky L, Khalaila R, Yeshaya N, Noronha A, Lindzen M, et al. Computational optimization of antibody humanness and stability by systematic energy-based ranking. Nature Biomedical Engineering. 2023;8(1):30–44. Available from: https://doi.org/10.1038/s41551-023-01079-1
2.
Hema Thanka Christlet T, Veluraja K. Database Analysis of O-Glycosylation Sites in Proteins. Biophysical Journal. 2001;80(2):952–60. Available from: https://doi.org/10.1016/s0006-3495(01)76074-2