Improvements in antigen binding are correlated with decreases in expression

Summary

Improvements in antigen binding by antibodies is correlated with decreases in expression (1). The exact magnitude of the correlation depends on models of substitution.

See also

• Stability-activity trade-off during enzyme design and evolution is highly local and not global

1.

Tharp CR, Catalano C, Khalifeh A, Ghaffari-Kashani S, Huang R, Kang G, et al. Biophysical trade-offs in antibody evolution are resolved by conformation-mediated epistasis. openRxiv; 2026. Available from: https://doi.org/10.64898/2026.03.12.711465