Interleukins are one of the major types of cytokine.

Ref (1)

Interleukin-1

A pyrogen produced by dendritic cells and macrophages in response to infection

Interleukin-2

  • Low doses stimulate regulatory T cells and high doses stimulate CD8+-cytotoxic T cells and Natural Killer cells.
  • Approved for metastatic renal cell carcinoma in 1992.
  • A high-affinity IL-2 was used to generate a partial agonist H9T that had reduced binding at H9T-IL2RG interface.
  • An IL-2/antibody conjugates can specifically activate CD8+-cytotoxic-T-cells. Masked versions have been created and tested.

Interleukin-6

A pyrogen. Initializes inflammation response along with IL-1 and TNF-. Also has various anti-inflammatory effects.

Interleukin-12

An IL-12/collagen-binding domain conjugate took advantage of high levels of collagen in the tumor microenvironment to preferentially target tumor sites. Masked versions have been created and tested.

Interleukin-18

Explored as a therapeutic, but low efficacy due to present of IL-18 binding protein, which is upregulated in tumor microenvironment. Decoy-resistant versions have been engineered by Zhou et al (Nature, 2020). Promotes cytokine production in T cells, but which types depend on the presence of IL-12 and/or IL-15.

Synthetic interleukins

A mimic of IL-2/IL-15 was made with Rosetta by Silva et al (Nature, 2019) that binds all three receptor chains. A second-generation version did not need to contact the alpha chain, and had greater thermostability.

1.
Aung T, Grubbe WS, Nusbaum RJ, Mendoza JL. Recent and future perspectives on engineering interferons and other cytokines as therapeutics. Trends in Biochemical Sciences. 2023;48(3):259–73. Available from: https://doi.org/10.1016/j.tibs.2022.09.005