Summary
Lower antibody affinity is desirable when target antigens are present in non-tumor tissues, albeit in lower concentrations (on-target off-tumor targeting; (1)).
1.
Weisser NE, Sanches M, Escobar-Cabrera E, O’Toole J, Whalen E, Chan PWY, et al. An anti-HER2 biparatopic antibody that induces unique HER2 clustering and complement-dependent cytotoxicity. Nature Communications. 2023;14(1). Available from: https://doi.org/10.1038/s41467-023-37029-3