Summary
Monomeric AlphaFold2 is better able to distinguish positive and negative PPIs than AF2-multimer (1). (2) found that this made it effective at filtering de novo binder designs made by hallucination.
Figures
Ref (1)
1.
Zhang J, Humphreys I, Pei J, Cong Q. Computing the human interactome. Structural Dynamics. 2025;12(5_Supplement):A176–A176. Available from: https://doi.org/10.1063/4.0000966
2.
Pacesa M, Nickel L, Schellhaas C, Schmidt J, Pyatova E, Kissling L, et al. One-shot design of functional protein binders with BindCraft. Nature. 2025;646(8084):483–92. Available from: https://doi.org/10.1038/s41586-025-09429-6