Summary

FR2 is more hydrophilic in nanobodies than in antibodies (1).

Figures

Ref (1)

1.
Ketaren NE, Fridy PC, Malashkevich V, Sanyal T, Brillantes M, Thompson MK, et al. Unique mechanisms to increase structural stability and enhance antigen binding in nanobodies. Structure. 2025;33(4):677-690.e5. Available from: https://doi.org/10.1016/j.str.2025.01.019