Summary
Missense mutations destabilize proteins on average by 1.3 kcal/mol (1), though (2) calculated at 0.22 kcal/mol.
1.
Tokuriki N, Stricher F, Serrano L, Tawfik DS. How Protein Stability and New Functions Trade Off. PLoS Computational Biology. 2008;4(2):e1000002. Available from: https://doi.org/10.1371/journal.pcbi.1000002
2.
Tsuboyama K, Dauparas J, Chen J, Laine E, Mohseni Behbahani Y, Weinstein JJ, et al. Mega-scale experimental analysis of protein folding stability in biology and design. Nature. 2023;620(7973):434–44. Available from: https://doi.org/10.1038/s41586-023-06328-6