Summary
Immunogenic reactions against VHH nanobodies disproportionately target their C-termini (1). The reasons were unclear as of 2021.
See also
1.
Rossotti MA, Bélanger K, Henry KA, Tanha J. Immunogenicity and humanization of single‐domain antibodies. The FEBS Journal. 2021;289(14):4304–27. Available from: https://doi.org/10.1111/febs.15809