Antibody thermostability is not correlated with hydrophobicity

Summary

Fab thermostability was not correlated with hydrophobicity (1).

Details

In a panel of B cell-derived mAbs, apparent melting temperature and HIC retention time had $R^2=0.0126$.

Figures

Figure S3B from (1)

See also

• Antibody thermostability decreases during affinity maturation, up to a threshold
• Somatic hypermutation correlates with lower hydrophobicity

1.

Shehata L, Maurer DP, Wec AZ, Lilov A, Champney E, Sun T, et al. Affinity Maturation Enhances Antibody Specificity but Compromises Conformational Stability. Cell Reports. 2019;28(13):3300-3308.e4. Available from: https://doi.org/10.1016/j.celrep.2019.08.056