Summary
Changes in the sequence of CDRs is sufficient to destabilize antibodies and lower thermostability (1). A single antibody framework can, with different CDRs, have melting temperatures ranging from 40° to 80° C.
1.
Wan Y, Liang J, Dai Y, Srinivasan K, Billesbølle C, Zhu J-F, et al. Hypervariable loop profiling decodes sequence determinants of antibody stability. Nature Structural & Molecular Biology. 2026 Apr; Available from: http://dx.doi.org/10.1038/s41594-026-01804-9