Summary
Directed evolution can uncover beneficial sequence modifications that might be missed by inverse folding (1). This was shown with a mid-helical alanine-to-proline mutation.
See also
1.
Hou K, Huang W, Qi M, Tugwell TH, Alturaifi TM, Chen Y, et al. De novo design of porphyrin-containing proteins as efficient and stereoselective catalysts. Science. 2025;388(6747):665–70. Available from: https://doi.org/10.1126/science.adt7268