Summary
Experimentally successful designed miniprotein binders tend to have higher predicted folding stability than unsuccessful designs. Models trained on megascale stability measurements improve discrimination between stable and unstable computationally designed proteins (1).
See also
- Protein structure prediction and design confidence metrics do not correlate with binding affinity
- Buried nonpolar surface area is a major determinant of whether de novo designed proteins are stable
1.
Cho Y, Tsuboyama K, Litberg TJ, Jung MD, Obisesan A, Wang Q, et al. Accurate protein stability prediction for small domains using mega-scale experiments. openRxiv; 2026. Available from: https://doi.org/10.64898/2026.05.19.726285