Summary
Heavy and light chains of scFvs with the same framework and antigen-binding mode can be exchanged to increase screening diversity (1). This was not screened in vitro but was observed using AlphaFold3 filtering.
Figures
Ref (1)
1.
Bennett NR, Watson JL, Ragotte RJ, Borst AJ, See DL, Weidle C, et al. Atomically accurate de novo design of antibodies with RFdiffusion. Nature. 2025;649(8095):183–93. Available from: https://doi.org/10.1038/s41586-025-09721-5