Summary
Sequence abundance from NGS does not correlate with binding affinity (1,2).
1.
Paul S, Harvey EP, Osei-Owusu J, Kollasch AW, Riesselman AJ, McMahon C, et al. Machine learning enables efficient and effective affinity maturation of nanobodies. openRxiv; 2026. Available from: https://doi.org/10.64898/2026.01.11.698911
2.
Makowski EK, Kinnunen PC, Huang J, Wu L, Smith MD, Wang T, et al. Co-optimization of therapeutic antibody affinity and specificity using machine learning models that generalize to novel mutational space. Nature Communications. 2022;13(1). Available from: https://doi.org/10.1038/s41467-022-31457-3