Antibody hydrophobicity sometimes correlates with polyreactivity

Summary

Antibody hydrophobicity and polyreactivity sometimes correlate, but that varies between datasets (1,2).

Details

In B cell-derived mAbs analyzed by Shehata et al., HIC retention time and PSR score were only weakly coupled ($R^2=0.1249$). A larger human-antibody study instead found that heavy-chain CDR hydrophobicity and positive charge were associated with polyreactivity (2).

Figures

Figure S3A from (1)

See also

• Somatic hypermutation correlates with lower hydrophobicity
• Somatic hypermutation correlates with lower polyreactivity

1.

Shehata L, Maurer DP, Wec AZ, Lilov A, Champney E, Sun T, et al. Affinity Maturation Enhances Antibody Specificity but Compromises Conformational Stability. Cell Reports. 2019;28(13):3300-3308.e4. Available from: https://doi.org/10.1016/j.celrep.2019.08.056

2.

Chen H-T, Zhang Y, Huang J, Sawant M, Smith MD, Rajagopal N, et al. Human antibody polyreactivity is governed primarily by the heavy-chain complementarity-determining regions. Cell Reports. 2024;43(10):114801. Available from: https://doi.org/10.1016/j.celrep.2024.114801