Summary
Long CDRH3 loops are enriched among polyreactive antibodies in some repertoires but are not strongly predictive on their own. Long, protruding CDRH3 loops characterize some polyreactive antibodies, but many polyreactive antibodies lack this feature (1). In a nanobody dataset, CDR3 length alone had near-random predictive performance for polyreactivity (2).
See also
1.
Lecerf M, Lacombe RV, Dimitrov JD. Polyreactivity of antibodies from different B-cell subpopulations is determined by distinct sequence patterns of variable region. Frontiers in Immunology. 2023;14:1266668. Available from: https://doi.org/10.3389/fimmu.2023.1266668
2.
Harvey EP, Shin J-E, Skiba MA, Nemeth GR, Hurley JD, Wellner A, et al. An in silico method to assess antibody fragment polyreactivity. Nature Communications. 2022;13(1):7554. Available from: https://doi.org/10.1038/s41467-022-35276-4