Summary
The CDR3 loop of nanobodies is far more likely to be part of the paratope than the CDRH3 of traditional heterodimeric antibodies ((1), De (2)). Additionally, it comprises a larger part of the paratope than CDRH3 in antibodies.
Figures
Ref De (2)
1.
Gordon GL, Capel HL, Guloglu B, Richardson E, Stafford RL, Deane CM. A comparison of the binding sites of antibodies and single-domain antibodies. Frontiers in Immunology. 2023;14. Available from: https://doi.org/10.3389/fimmu.2023.1231623
2.
De Genst E, Silence K, Decanniere K, Conrath K, Loris R, Kinne J, et al. Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodies. Proceedings of the National Academy of Sciences. 2006;103(12):4586–91. Available from: https://doi.org/10.1073/pnas.0505379103