Ancestral sequence reconstruction refers to the process of inferring ancestral sequences using extant sequences.
Figure from (1)
1.
Dishman AF, Tyler RC, Fox JC, Kleist AB, Prehoda KE, Babu MM, et al. Evolution of fold switching in a metamorphic protein. Science. 2021;371(6524):86–90. Available from: https://doi.org/10.1126/science.abd8700